Peptide Research Protocols by Janet J Maguire ISBN 9781617372919
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Peptide Research Protocols by Janet J Maguire ISBN 9781617372919

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Peptide Research Protocols by Janet J Maguire

The endothelins are a remarkable family of signaling peptides: molecular biology predicted the existence of their receptors and synthetic enzymes prior to both the identification of the encoded proteins and the synthesis of antagonists and inhibitors for use as pharmacological tools. Although considerable advances have been made, culminating in the design of endothelin antagonists with the- peutic potential in cardiovascular disease, much remains to be discovered. Tantalizingly, new research frontiers are emerging. To support further progress, Peptide Research Protocols: Endothelin encompasses experimental protocols that interrogate all facets of an endogenous mammalian peptide s- tem, from peptide and receptor expression through synthetic pathway to peptide function and potential role in human disease. Chapters describe the use of molecular techniques to quantify the expression of mRNA for both endothelin receptors and the endothelin-converting enzymes. Peptides, precursors, receptors, and synthetic enzymes may be localized and quantified in plasma, culture supernatants, tissue homogenates, and tissue s- tions using antibodies, while additional information on receptor characterization may be obtained using radioligand binding techniques. Several protocols cover in vitro assays that determine the function of the endothelin peptides in isolated preparations, that characterize new endothelin receptor ligands, or provide inf- mation on the tissue-specific processing of endothelin precursor peptides.

Book details

SKU NLS9781617372919
ISBN 13 9781617372919
ISBN 10 1617372919
Author Janet J Maguire
Series Methods In Molecular Biology
Condition New
Binding Type Paperback
Publisher Humana Press Inc.
Year published 2010-11-10
Number of pages 242
Cover note Book picture is for illustrative purposes only, actual binding, cover or edition may vary.

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