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| Language | English |
| Format | PDF (Premium Digital) |
| Ownership | Permanent download — yours forever |
Hydrocarbon and Lipid Microbiology Protocols by Terry J Mcgenity
This Volume presents a comprehensive series of generic protocols for the genetic and genomic analysis of prokaryotic isolates. Genetic methods for functional analyses employ the latest cloning vectors, gene fusion methods and transposon mutagenesis systems, as well as systems for introducing protease-cleavage sequences into permissive sites in proteins under investigation. Genomic methods described include protocols for transcriptomics, shotgun proteomics, interactomics, metabolic profiling, and lipidomics. Bioinformatic tools for genome annotation, transcriptome display and the integration of transcriptomic data into genome-scale metabolic reconstructions are described. Protocols for 13C-based metabolic flux determinations and analysis of the hierarchical and metabolic regulation of fluxes through pathways are included. The Volume thus enables investigators to functionally analyse an isolate over the entire cellular range spanning the gene, the genome, the transcript repertoire, the proteome, the interactome, the metabolic network with its nodes and their regulatory hierarchies, and the metabolic fluxes and their physiological controls.
Hydrocarbon and Lipid Microbiology Protocols
| ISBN 13 | 9783662570593 |
|---|---|
| ISBN 10 | 3662570599 |
| Author | Terry J Mcgenity |
| Series | Springer Protocols Handbooks |
| Condition | New |
| Binding Type | Paperback |
| Publisher | Springer |
| Year published | 2018-09-09 |
| Number of pages | 258 |
| Cover note | Book picture is for illustrative purposes only, actual binding, cover or edition may vary. |
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