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| Language | English |
| Format | PDF (Premium Digital) |
| Ownership | Permanent download — yours forever |
Human Cancer in Primary Culture, A Handbook by John Masters
Growing human cancer cells in primary culture requires patience, intuition, care and experience. This is one of the few areas where the wrinkled senior scientist can be more productive than the bright young post-doc. There are few mechanical aids, no automated procedures, and kits are unheard-of. There is no right way to do it and every tumour is different. But this book will make it easier Chapter 1 on characterization is essential reading. Much published work is useless because of the failure to take two simple steps to characterise the cells. The first step is to fully record all the clinical data - the absence of this information can render the work valueless. The second step is to confirm the origin of the tissue to exclude cross- contamination. The wastage of years of work can be avoided with the use of a simple DNA preparation with a couple of commercially-available probes. Chapter 2 describes the development of serum-free media. This is a goal many would like to achieve, particularly if someone else does it, as it is laborious and empirical. Defined serum-free medium is essential for studies of growth factors and has major advantages in the commercial preparation of cell products and other applications.| ISBN 13 | 9789401054591 |
|---|---|
| ISBN 10 | 9401054592 |
| Author | John Masters |
| Series | Developments In Oncology |
| Condition | New |
| Binding Type | Paperback |
| Publisher | Springer |
| Year published | 2012-10-31 |
| Number of pages | 299 |
| Cover note | Book picture is for illustrative purposes only, actual binding, cover or edition may vary. |
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"I compared Kindle vs this store. Kindle is cheaper monthly but you don't own anything. Here I paid once and the PDF is mine for life. Highly recommend."
"Left Kindle behind after they removed a book I was reading mid-series. With this PDF, nobody can take it away from me. True ownership feels good."
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"My Kindle subscription was getting expensive. Switched to buying PDFs here and I'm saving money while actually building a permanent digital library I control."
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